Cat. No.
MABL-2294
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Chicken
Clone No.
D1.3
From
Recombinant Antibody
Specificity
This antibody specifically binds hen egg-white lysozyme.
Alternative Names
HEL; Hen Egg white Lysozyme; Lysozyme C; 1,4-beta-N- acetylmuramidase C; Allergen Gal d IV; Allergen: Gal d 4
UniProt
P00698
Immunogen
The original antibody was generated by immunizing BALB/c mice with hen egg white lysozyme.
Application Notes
Lysozyme is an enzyme that degrades polysacchardies of various types of bacterial cell walls. It is observed that lysozyme bound D1.3 still retains its enzymatic activity. This is attributed to the fact that D1.3 binds lysozyme at a site remote from the catalytic site and that there is no significant conformational change caused by the binding (PMID: 3614217). The three dimension structure of a complex between the Fab from antibody D1.3 and its antigen, HEL, has been determined at 6 and at 2.8 a resolution (PMID: 2426778, 3965976). Sixteen residues of lysozyme make contact with 17 residues of the antibody. The antigenic determinant is topographic including lysozyme residues 18, 19, 21–25, 27, 116–121, 124, and 129. All six complementarity determining regions of the antibody contact the antigen, and in addition, two residues from “framework” segments of V H and V L (Amit et al., 1986; PMID: 2441250). D1.3 binds native lysozyme in an ELISA (PMID: 2448386). Humanized version of this antibody is also available (PMID: 1560463, 2451287). Flow cytometry was used to find the fine difference in affinity of D1.3 (scFv) and mutant M3 clone expressed in a yeast surface display (PMID:10662486).
Antibody First Published
Mariuzza et al. Preliminary Crystallographic Study of the Complex Between the Fab Fragment of a Monoclonal Anti-Lysozyme Antibody and Its Antigen. J Mol Biol, (1983) 15;170(4):1055-8. PMID:6644814
Note on publication
Describes the crystallographic study of the monoclonal antibody bound to lysozyme.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
