Cat. No.
MABL-2298
Application
HA, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Streptococcus pyogenes, Human
Clone No.
27.4.1
From
Recombinant Antibody
Specificity
This antibody reacts with α-helical molecules. Specifically, it binds streptococcal M-protein (type 5), human myosin, keratin, tropomyosin, ganglioside, and glycophorin M,N.
Alternative Names
Myosin; Streptococcal M-protein; Keratin; Tropomyosin; Ganglioside; Glycophorin M,N
UniProt
P02977
Immunogen
The original antibody was generated by immunizing BALB/c mice with streptococcal M type 5 membranes followed by a second, boosting dose of Lysin M5.
Application Notes
This antibody reacts with the human retina, as shown in an indirect immunofluorescence assay. This antibody reacts with retinal S-antigen (S-Ag), as shown in an ELISA (Lerner et al., 1995; PMID: 8722579). This antibody binds myosin at a high relative avidity, as determined in a competitive ELISA. Reactivity with glycophorin M and N was determined by hemagglutination assay (Mertens et al., 2000; PMID: 11282394).
Antibody First Published
Cunningham et al. Human and murine antibodies cross-reactive with streptococcal M protein and myosin recognize the sequence GLN-LYS-SER-LYS-GLN in M protein J Immunol. 1989 Oct 15;143(8)：2677-83 PMID:2677144
Note on publication
The original publication describes the use of overlapping synthetic peptides to map the myosin cross-reactive epitopes of streptococcal M protein recognized by antibodies from acute rheumatic fever and rheumatic heart disease sera.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
