Cat. No.
MABL-2299
Application
HA, PRNT, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Coxsackievirus, Poliovirus type 1, Rat, Streptococcus pyogenes, Human
Clone No.
36.2.2
From
Recombinant Antibody
Specificity
This antibody reacts with α-helical molecules. Specifically, it binds streptococcal M-protein (type 5), human myosin, actin, keratin, tropomyosin, troponin, laminin, ganglioside, glycophorin M,N
Alternative Names
Streptococcal M-protein; Myosin; Actin; Keratin; Tropomyosin; Troponin; Laminin; Ganglioside; Glycophorin M,N
UniProt
P02977
Immunogen
The original antibody was generated by immunizing BALB/c BYJ mice with streptococcal M type 5 membranes followed by a second, boosting dose of M type 5 streptococcal membranes.
Application Notes
This antibody reacts with human heart cells and whole streptococci (group A, M type 5), as shown in an ELISA, and human heart cells, human sarcolemma, and group A streptococcal membranes, as shown in a WB (Cunnigham et al., 1984; PMID: 6384047). This antibody reacts with the heavy chain of skeletal muscle myosin. Specifically, it reacts with light meromyosin fragments, as shown in WB and competitive inhibition assays (Cunningham et al., 1986; PMID: 3510006). This antibody labels the cytoplasm of epidermal cells and epidermal appendages in normal and psoriatic skin, as shown by immunofluorescent staining (Swerlick et al., 1986; PMID: 3525693).
Antibody First Published
Cunningham et al. Murine monoclonal antibodies reactive with human heart and group A streptococcal membrane antigens Infect Immun. 1984 Oct;46(1):34-41. doi:10.1128/iai.46.1.34-41.1984 PMID:6384047
Note on publication
The original publication characterizes the antibody's reactivity with human heart streptococcal antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
