Cat. No.
MABL-2300
Application
HA, PRNT, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Rat, Streptococcus pyogenes, Human
Clone No.
49.8.9
From
Recombinant Antibody
Specificity
This antibody reacts with α-helical molecules and the carbohydrate N-acetyl-b-D-glucosamine (GlcNac). Specifically, it binds streptococcal M-protein (type 5), human myosin, keratin, DNA, vimentin, troponin, ganglioside, and glycophorin M,N.
Alternative Names
Myosin; Streptococcal M-protein; Keratin; DNA; Vimentin; Troponin; Ganglioside; Glycophorin M,N
UniProt
P02977
Immunogen
The original antibody was generated by immunizing BALB/c BYJ mice with streptococcal M type 5 membranes followed by a second, boosting dose of M type 5 streptococcal membranes.
Application Notes
This antibody reacts with human heart cells and whole streptococci (group A, M type 5), as shown in an ELISA, and with human heart cells, human sarcolemma (weak response), and group A streptococcal membranes (weak response), as shown in a WB (Cunnigham et al., 1984; PMID: 6384047). This antibody labels the cytoplasm of epidermal cells and epidermal appendages in normal and psoriatic skin, as shown by immunofluorescent staining (Swerlick et al., 1986; PMID: 3525693). This antibody binds CV (B3), as shown in an ELISA; neutralizes PV-1 and CV (B3), as shown in a plaque-reduction neutralization assay; and is slightly cytotoxic to rat fibroblasts, as shown in a cytotoxicity assay (Cunningham et al., 1992; PMID: 1311095). This antibody reacts with retinal S-antigen (S-Ag), as shown in an ELISA (Lerner et al., 1995; PMID: 8722579). This antibody binds myosin at a medium relative avidity, as determined in a competitive ELISA. Reactivity with glycophorin M and N was determined by hemagglutination assay (Mertens et al., 2000; PMID: 11282394).
Antibody First Published
Cunningham et al. Murine monoclonal antibodies reactive with human heart and group A streptococcal membrane antigens Infect Immun. 1984 Oct;46(1):34-41. doi:10.1128/iai.46.1.34-41.1984 PMID:6384047
Note on publication
The original publication characterizes the antibody's reactivity with human heart and streptococcal antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
