Cat. No.
MABL-2314
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Baboon, Pigtailed Macaque, rhesus macaque, Cynomolgus Monkey
Clone No.
1B3
From
Recombinant Antibody
Specificity
This antibody reacts with rhesus macaque IgG1 and IgG3, as well a cynomolgus monkey, pigtailed macaque and baboon IgG. It has minimal reactivity with human, mangabey, vervet, chimpanzee, or marmoset IgG.
Alternative Names
Macaque IgG; Macaque immunoglobulin G
UniProt
Immunogen
This antibody was raised by immunising mice with baboon IgG and screening hybridomas against IgG of different primate species.
Application Notes
This anti-macaque antibody has been used in ELISA analysis to detect rhesus anti- mouse antibodies in serum samples (Page et al, 2012). As this antibody is able to distinguish between human and macaque IgG, it has also been used in ELISA analysis to detect macaque antibodies raised against intravenously-administered human HIV broadly neutralising antibodies (Rosenberg et al, 2015). Additionally, it has been used in flow cytometric analysis to confirm the clearance of intravenously- administered anti-CD4 depleting antibody from the plasma of rhesus macaques (Mudd et al, 2011).
Antibody First Published
Mudd et al. Reduction of CD4+ T cells in vivo does not affect virus load in macaque elite controllers. J Virol. 2011 Jul;85(14):7454-9. PMID:21593153
Note on publication
This antibody is from the laboratory of Keith Reimann, DVM, Beth Israel Deaconess Medical Center.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
