Cat. No.
MABL-2313
Application
FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
YBM15.1
From
Recombinant Antibody
Specificity
This antibody recognizes murine integrine MAC-1 (Itgam/Cd11b). This integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens.
Alternative Names
CD11b; Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; YBM 15.1.6; YBM15.1.6
UniProt
P05555
Immunogen
This antibody was derived from fusions between the 210 RC Y3/Ag 1.2.3 rat myeloma cell line and spleen cells from rats hyperimmunized with adult mouse bone marrow.
Application Notes
This antibody is recommended for the detection and analysis of MAC-1, the murine equivalent of CD11b. For instance, YBM15.1 was used in a panel of antibodies in the assessment of the presence of antigenic determinants on normal erythroid progenitors via flow cytometry (Watt et al., 1987; pmid: 3330997). This antibody was used to detect the integrin MAC-1 in the neural tissue of Biozzi ABH mice infected with Semliki Forest virus (SFV) A7(74) via immunohistochemistry (Smith et al., 2000; pmid: 10814783).
Antibody First Published
Watt et al. Cell-surface markers on haemopoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations Mol Cell Probes. 1987 Dec;1(4):297-326. doi: 10.1016/0890-8508(87)90013-2. PMID:3330997
Note on publication
The article describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
