Cat. No.
MABL-2312
Application
FC, IF
Isotype
Engineer antibody
Species Reactivity
Influenza A
Clone No.
C2
From
Recombinant Antibody
Specificity
The antibody is specific for M2-cytoplasmatic domain. M2 is a 97-amino acid protein of which approximately half (aa 47–97) comprises the cytoplasmic domain. M2 is highly conserved among virus strains.
Alternative Names
M2 protein; M; Matrix protein 2; Proton channel protein M2; M2-E10
UniProt
P05780
Immunogen
The original antibody was generated by phage display and yeast display based antibody selection and screening. The purified M2 cytodomain was biotinylated and used as a target for phage display-based single-chain variable fragment (scFv) antibody selection. After two rounds of phage selection, scFv genes were cloned into a yeast display vector, and the yeast display library was sorted for binding.
Application Notes
The scFv-Fc version of the antibody was tested against HEK cells expressing full-length M2 protein. Fluorescent signals were visible lining the internal cell wall of HEK M2 cells after fixation and permeabilization. No signal was visible in HEK cells that do not express M2, or when the cells were not permeabilized. The ability of the antibody to recognize native M2 protein produced during IVA infection of MDCK cells with the H3N2 (A/PCh/1/73) and H1N1 (A/PR/8/34) strains of influenza was detected. Efficient and specific recognition of native M2 protein by the scFv-Fc fragment of the antibody was observed via fluorescent microscopy and flow cytometry (Velappan et al., 2020; PMID: 33206590).
Antibody First Published
Velappan et al. Selection and verification of antibodies against the cytoplasmic domain of M2 of influenza, a transmembrane protein MAbs. Jan-Dec 2020;12(1):1843754. PMID:3320659
Note on publication
The original paper describes the generation of the original antibody, using phage and yeast display selection techniques.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
