Cat. No.
MABL-2322
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
cattle, Deer, Horse, Pig, Sheep
Clone No.
6G1
From
Recombinant Antibody
Specificity
This antibody is specific for mammalian skeletal Troponin I (sTnI) and can cross-react with skeletal Troponin C (sTnC).
Alternative Names
Skeletal Troponin I; Troponin I; skeletal Troponin C; sTnC
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with heat-treated TnI in Freund's complete adjuvant, followed by two booster injections with TnI in Freund's incomplete adjuvant.
Application Notes
This antibody was originally reported to react with equine (horse) Troponin I (TnI) as determined by ELISA (US7297500B2). Later, it was shown through WB to bind mammalian skeletal TNI (sTnI), specifically bovine (cow) and porcine (pig), where it was shown to be immunoreactive to both sTnI and skeletal Troponin C (sTnC). However, sTnC was not detected by this antibody following extraction using urea and βME supplemented extraction buffer, indicating that their addition leads to the destruction of the antibody's epitope on sTnC. In a sandwich ELISA, it was determined that this antibody detects heated bovine, cervine (deer), equine, ovine (sheep), and porcine samples (Jiang et al., 2020; DOI: 10.1016/j.foodcont.2019.107045).
Antibody First Published
Hsieh et al. Use of troponin I as a species marker protein for meat speciation in both raw and heat-processed products. US7297500
Note on publication
The original publication describes the development and applications of monoclonal antibodies which bind to heat-treated proteins of meat.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
