Cat. No.
MABL-2323
Application
crystallography, in vitro, in vivo, ITC, ELISA
Isotype
Engineer antibody
Species Reactivity
Staphylococcus aureus, Staphylococcus epidermidis
Clone No.
305-78-7
From
Recombinant Antibody
Specificity
The antibody binds to a conformational epitope on MntC located between K243 and E260. The epitope involves α-helix α8, β-sheet β8 and a short loop connecting these two structural elements. The antibody interferes with manganese binding to the protein. Staphylococcus aureus protein MntC is a metal- binding protein of the ABC-type transporter involved in the acquisition of Manganese (II) ions.
Alternative Names
MntC; ABC transporter substrate-binding protein; Manganese ABC transporter substrate-binding lipoprotein; Manganese ABC transporter substrate-binding protein; Manganese-binding lipoprotein mntA; Manganese-binding protein; Metal ABC transporter substrate-binding protein; Periplasmic solute binding protein
UniProt
W8TNQ9
Immunogen
The original antibody was generated by immunizing mice with purified recombinant MntC.
Application Notes
The antibody showed reduce S. aureus burden in an infant rat passive protection model and induced neutrophil respiratory burst activity against both Staphyloccocus aureus and Staphyloccocus epidermidis (Anderson et al., 2012; PMID: 22474033). The structure of the antibody in complex with MntC was determined. Epitope mapping was carried out using Hydrogen-Deuterium Exchange Mass Spectrometry, the results were further validated using X-ray crystallography, site-directed mutagenesis and functional studies. The binding of the antibody to MntC was tested using ITC and ELISA. ITC experiments showed that binding of the wild type MntC to the antibody was stoichiometric, the apparent Kd was 1.2 nM, and the binding enthalpy was -19.9 kcal/mol. The specificity of the antibody was confirmed by ELISA analysis, with Kd= 1.3 nM. (Gribenko et al., 2016; PMID: 27689696)
Antibody First Published
Anderson et al. Staphy.lococcus aureus manganese transport protein C is a highly conserved cell surface protein that elicits protective immunity against S. aureus and Staphylococcus epidermidis J Infect Dis. 2012 Jun;205(11):1688-96. PMID:22474033
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
