Cat. No.
MABL-2324
Application
ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Mycobacterium bovis, Mycobacterium malmoense, Mycobacterium marinum, Mycobacterium scrofulaceum, Mycobacterium simiae, Mycobacterium tuberculosis, Mycobacterium xenopi
Clone No.
My2F12
From
Recombinant Antibody
Specificity
This antibody is specific for α1-2 linkage of the mannose-capped lipoarabinomannan (LAM) of Mycobacterium tuberculosis and is capable of distinguishing ManLAM from two different M. tuberculosis strains from PILAM of M. smegmatis in sandwich ELISA on both spiked PBS and urine samples. This antibody can only bind mannose oligosaccharides containing the terminal linear α1-2 mannose linkages and not mannose oligosaccharides or phosphoinositol mannosides (PIMs) with only α1-4 or α1-6 mannose linkages or the branched α1-2, 6 linkages on hexamannan.
Alternative Names
Lipoarabinomannan; Lipoarabinomannan carrier protein LprG; 27 kDa lipoprotein; Antigen P27; Lipoprotein LprG; Triacylglyceride transfer protein LprG
UniProt
P9WK45
Immunogen
The original antibody was isolated from a non-immune human Fab-antibody phage display library by multiple rounds of screening against Mycobacterium tuberculosis mannose-capped lipoarabinomannan (LAM) combined with negative depletion on M. smegmatis PILAM.
Application Notes
This antibody is recommended for the detection of M. tuberculosis lipoarabinomannan (LAM) using ELISA. The binding affinity of the human IgG1 version of this antibody for mannose-capped lipoarabinomannan was found to be Kd= 0.438 nM. This antibody was reported to bind mannose-capped LAM of all the slow-growing pathogenic Mycobacterium species. This antibody can also be used for the immunofluorescent staining of heat-killed M. tuberculosis bacilli fixed by methanol–acetone onto slides. This antibody can be used for the detection of tuberculosis (TB) infection via IF sputum smears. This antibody was also used in the detection of mannose-capped LAM in TB patients' serum and urine samples (PMID: 25975873).
Antibody First Published
Chan et al. The diagnostic targeting of a carbohydrate virulence factor from M. tuberculosis. Sci Rep. 2015 May 15;5:10281. PMID:25975873
Note on publication
This paper describes the use of this antibody in the development of a diagnostic assay for detection of mannose capped LAM in patient's urine and serum samples.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
