Cat. No.
MABL-2348
Application
IP, SPR, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MN-1
From
Recombinant Antibody
Specificity
This antibody can recognize and bind human mesothelin. This antibody binds a different antibody on the human mesothelin than another antibody called MB. Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein highly expressed in several types of malignant tumors sometimes in association with increased tumor aggressiveness and poor clinical outcome. The membrane-anchored forms of this molecule may play a role in cellular adhesion.
Alternative Names
MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; MN
UniProt
Q13421
Immunogen
The original antibody was generated in mesothelin-deficient mice by immunization with plasmid cDNA corresponding to the mesothelin extracellular domain followed by a single boost with a recombinant human mesothelin-rabbit IgG Fc fusion protein.
Application Notes
The binding characterization of this antibody was done using ELISA and SPR. The binding affinity of the original IgG2a antibody for mesothelin-Fc protein was determined by BIACore and reported to be Kd = 1.0 nM. This antibody can recognize membrane bound mesothelin on the surface of mesothelin positive H226, A1847, Hela and Panc3.014 cells (mesothelin-positive) in a flow cytometry. This antibody can also bind denatured human mesothelin in a western blot assay. This antibody was also used in the immunohistochemical staining of cancerous tissue like ovarian adenocarcinoma and normal tissues like peritoneal mesothelium on uterus, pleural mesothelium on lung and fallopian tube luminal fluid (PMID: 16115924). This antibody was also used in an immunohistochemical analysis of 1562 tumors for mesothelin expression. It was reported that mesothelin was highly expressed in ovarian serous carcinoma, pancreatic adenocarcinoma and malignant pleural mesothelioma (PMID: 28460459). This antibody was also used in the immunoprecipitation of mesothelin from cell lysates (PMID: 23694968).
Antibody First Published
Onda et al. New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clin Cancer Res. 2005 Aug 15;11(16):5840-6. PMID:16115924
Note on publication
Describes the generation of two antibodies against different epitopes of human mesothelin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
