Cat. No.
MABL-2353
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
R3.04
From
Recombinant Antibody
Specificity
This antibody binds methotrexate, formerly known as amethopterin. It is a chemotherapy agent and immune-system suppressant. It is often used in treatment of cancer, autoimmune diseases, ectopic pregnancy and for medical abortions.
Alternative Names
MTX; amethopterin; 4-amino-N10-methylpteroylglutamic acid; 4-amino- 10-methylfolic acid; CHEBI:44185; CAS number: 59-05-2
UniProt
Immunogen
The original antibody was generated by immunizing a female llama with methotrexate–BIP conjugate.
Application Notes
The binding reactivity of this single chain antibody for methotrexate-BSA was done using direct and competitive ELISA, with no cross reactivity with only BSA. The binding affinity of the original VHH version of this antibody as determined by BIAcore analysis was reported to be Kd=29 nM (PMID: 17011035).
Antibody First Published
Rueda et al. Generation of llama single-domain antibodies against methotrexate, a prototypical hapten. Mol Immunol. 2007 Mar;44(7):1680-90. PMID:17011035
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
