Cat. No.
MABL-2352
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Bovine
Clone No.
K42-IL
From
Recombinant Antibody
Specificity
The antibody binds specifically to the C3-loop of metarhodpsin-I with a Kd of 2.8 nM, the epitope being TGALQERSK.
Alternative Names
UniProt
P02699
Immunogen
Bovine rod cell disc membranes.
Application Notes
This antibody specifically binds to the C3-loop of metarhodopsin-I, which is in equilibrium with the meta-active metarhodopsin II. The antibody stabilises the inactive metarhodopsin-I, inhibiting G-protein activation.
Antibody First Published
Adamus et al. Use of peptides to select for anti-rhodopsin antibodies with desired amino acid sequence specificities. Peptide Research 1988; 1(1):42-47 PMID:2485225
Note on publication
Describes the generation of several monoclonal antibodies against rhodopsin using peptides for hybridoma selection.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
