Cat. No.
MABL-2364
Application
IP, FC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-27
From
Recombinant Antibody
Specificity
This antibody recognizes a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
Alternative Names
Major Histocompatibility Complex 1; MRC OX27; OX27
UniProt
Immunogen
This antibody was prepared by immunizing BALB/c mice with rat phytohemagglutinin blasts.
Application Notes
This antibody binds to rat MHC Class I, and has been used to show that, in an in vivo rat model, antibodies against MHC class I antigens from stored blood may precipitate transfusion-related acute lung injury (Kelher, 2009).
Antibody First Published
Jeffries et al. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med. 1985 Jul 1;162(1):117-27. PMID:3159821
Note on publication
Describes the original use of this antibody in immunoprecipitations to generate an MHC I precipitate for use in comparative SDS page analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
