Cat. No.
MABL-2369
Application
radioimmunoassay
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
A6-61.3
From
Recombinant Antibody
Specificity
This antibody is specific for murine I-A, haplotypes H-2k, d and q. It binds to the β-chain of murine Ia.
Alternative Names
A6.61.3; I-A; Ia; I-E; MHC-II; MHC Class II
UniProt
Immunogen
This antibody was obtained from a fusion of the non-producing plasmacytoma Ag8.653 with spleen cells from ATL mice that had been immunized with ATH spleen cells.
Application Notes
This antibody has been used in a cellular radioimmunoassay to investigate the effect of IFN-y treatment on cell surface expression of Ia (Graf et al, 1985).
Antibody First Published
Koch et al Ia antigens and associated invariant chain are induced simultaneously in lines of T-dependent mast cells by recombinant interferon-gamma. J Immunol. 1984 Mar;132(3):1361-9. PMID:6420464
Note on publication
Describes the original generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
