Cat. No.
MABL-2370
Application
ICC, IP, RIA, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
OX-6
From
Recombinant Antibody
Specificity
This antibody recognises a monomorphic determinant of the rat MHC II (I-A) antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. It also cross-reacts with mouse MHC, specifically I-A[k] and I-A[s]
Alternative Names
MHC Class II; MRC OX-6; CD74; MRC OX 6; Major Histocompatibility complex II
UniProt
Immunogen
This antibody was prepared by subcutaneously injecting non-polymorphic MHC class II (Ia-like glycoproteins) from the membranes of rat thymocytes into BALB/c mice.
Application Notes
This antibody binds to the rat MHC II (I-A) antigen, and cross-reacts with mouse I-A[k] and I-A[s]. Consequently, this antibody can be used to assess the tissue distribution of rat Ia antigens, indicating high Ia antigen expression on peripheral B lymphocytes, and significantly lower expression on thymocytes and T lymphocytes (McMaster, 1979).
Antibody First Published
McMaster et al. Identification of Ia glycoproteins in rat thymus and purification from rat spleen. European Journal of Immunology. 1979, 9: 426-433 PMID:315315
Note on publication
Describes the original use of this antibody to identify and purify rat Ia glycoproteins, using indirect binding assays, FC and IP.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
