Cat. No.
MABL-2375
Application
Blocking, in vitro, in vivo, IP, SPR, FC, IF, Imaging
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
VHH7
From
Recombinant Antibody
Specificity
This antibody is specific for mouse MHCII.
Alternative Names
class II MHC; class II histocompatibility antigen
UniProt
Immunogen
The original antibody was generated by immunizing an alpaca with intact, unfractionated mouse splenocytes seven times.
Application Notes
This nanobody was linked to an anti-GFP VHH via their C termini to create a bispecific antibody capable of targeting both GFP and MHC class II Molecules; the VHH7-GFP bispecific construct effectively targeted B cells and facilitated the uptake of GFP by these cells, as indicated by a flow cytometry (FC) analysis that showed that most CD19+ cells were GFP+, indicating successful capture of GFP in vivo.The same bispecific antibody was used to stain B cells in mouse lymph node cells, as observed using FC. This nanobody was also used to C-terminally conjugate human IL2 and IFN-α to anti-GFP, demonstrating the general applicability of the approach for linking different entities (Witte et al., 2012; PMID
Antibody First Published
Duarte et al. Generation of Immunity against Pathogens via Single-Domain Antibody-Antigen Constructs J Immunol. 2016 Dec 15;197(12):4838-4847. doi: 10.4049/jimmunol.1600692 PMID:27821668
Note on publication
The original publication describes the development and application of single-domain antibody fragments (VHHs), such as VHH7, as targeted antigen-delivery vehicles for enhancing immune responses in antigen-presenting cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
