Cat. No.
MABL-2376
Application
IP, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
29C11
From
Recombinant Antibody
Specificity
This antibody binds the extracellular peptide called the Migis-α consisting of 26-32 amino acids towards the N-terminal of the human membrane bound IgA, when the lipid rafts are disrupted.
Alternative Names
Migis-α; mIgA isotype-specific extracellular peptide; extracellular membrane proximal domain of mIgA α chain
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with a mouse/human chimeric migis-αL-containing protein.
Application Notes
Membrane bound IgA is associated with Igα/Igβ as the B cell receptor (BCR) complex on mIgA-expressing B cells. The α chain of mIgA (mα) contains an extracellular domain that is reported to be an antigenic site suitable for isotype-specific targeting of mIgA-expressing B cells by antibodies. The mAb binds strongly to synthetic peptides of migis-α and to various recombinant proteins containing migis-α as revealed by ELISA. Flow cytometric analysis with B cells suggested that the mAb did not bind strongly to mIgA and required a higher concentration for detection. Immunoprecipitation analysis of these mAbs indicated that mIgA could only be pulled down by 29C11 when mIgA-expressing B cells were solubilized by strong detergents, such as sodium dodecyl sulfate (SDS) or when lipid rafts are disrupted (PMID: 21723611).
Antibody First Published
Hung et al. Lipid rafts hinder binding of antibodies to the extracellular segment of the membrane-anchor peptide of mIgA. Molecular Immunology (2011) Sep ;48 (15-16):1975-82. PMID:21723611
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
