Cat. No.
MABL-2378
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
04CT2.2.3
From
Recombinant Antibody
Specificity
This antibody binds auristatin (MMAE, MMAF) which is a cytotoxic drug (payload) employed in the ADC field. Monomethyl auristatin E (MMAE, vedotin) is a very potent antimitotic agent that inhibits cell division by blocking the polymerization of tubulin. The family of auristatins are synthetic analogues of the antineoplastic natural product Dolastatin 10, ultrapotent cytotoxic microtubule inhibitors that are clinically used as payloads in antibody-drug conjugates. As an antibody-drug conjugate or ADC, MMAE is linked to a monoclonal antibody (mAb) that recognizes a specific marker expression in cancer cells and directs MMAE to a specific, targeted cancer cell.
Alternative Names
Monomethylauristatin E; Vedotin; USAN; monomethyl auristatin F; NMAF; auristatins
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with MMAE-cysteine-KLH conjugate in equal volume of complete Freund's adjuvant.
Application Notes
The binding activity of this antibody towards MMAE-BSA conjugate was determined using ELISA. This antibody is also capable of binding to MMAE-conjugated antibody drugs and can be used for their detection in cancer research (CN114181307).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
