Cat. No.
MABL-2391
Application
functional assays, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
JF2 (1E2-A5-A3)
From
Recombinant Antibody
Specificity
This antibody is specific for the G3 domain of mouse nidogen-1. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Alternative Names
Nidogen-1; Nid1; Entactin
UniProt
P10493
Immunogen
The antibody was raised by immunizing fisher rats with recombinant mouse nidogen-1.
Application Notes
To characterize the original mouse version of this antibody ELISA was preformed against recombinant mouse nidogen-1 (Ries et al, 2001; pmid:11589703). Futhermore binding assays on mouse nidogen-1 were also preformed using the mouse version of this antibody (Ries et al, 2001; pmid:11589703). And finally this antibody was tested using surface plasmon resonance with mouse nidogen-1 as antigen (Ries et al, 2001; pmid:11589703). A tube-like formation assay was preformed on HMEC-1 cells. To study the influence of nidogen-1 in the process the mouse version of this antibody was used (Titz et al, 2004; pmid:15241558).
Antibody First Published
Ries et al. Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies Eur J Biochem. 2001 Oct;268(19):5119-28. PMID:11589703
Note on publication
The basement membrane protein, nidogen-1, was previously shown to consist of three globular domains, G1 to G3, and two connecting segments. Nidogen-1 is a major mediator in the formation of ternary complexes with laminins, collagen IV, perlecan and fibulins. In the present study, recombinant proteins of these predicted domains in mammalian cells were produced and used for crystallographic and binding epitope analyses.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
