Cat. No.
MABL-2392
Application
SPR, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
JF3 (4H5-B2-E4)
From
Recombinant Antibody
Specificity
This antribody is specific for the G2 domain of nidogen-1. It binds in such a way that it is able to stop the binding of nidogen to laminin. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Alternative Names
Nidogen-1; Nid1; Entactin; NID-1
UniProt
P10493
Immunogen
This antibody was raised by immunizing a rat with G1/link region of mouse nidogen that is able to block its binding to laminin.
Application Notes
The binding of the mouse version of this antibody to nidogen-1 was proven by ELISA. Further, high binding affinity of the mouse version of this antibody to nidogen-1 was demonstrated using surface plasmon resonance (Ries et al, 2001; pmid:11589703). Frozen sections of fetal, newborn, and 4- week-old mice were stained using the mouse version of this antibody to use for immunohistochemistry. This was performed while researching Collagen XXVIII (Veit et al, 2006; pmid:16330543). Frozen and paraffin- embedded sections of fetal, newborn, 3-week-old, and adult mice were stained using the mouse version of this antibody to use for immunohistochemistry. This assay was preformed while researching a protein called AMACO (Sengle et al, 2003; pmid:14506275). The mouse version of this antibody was used to stain whole mouse embryos or dissected organs from mice for immunohistochemistry. The staining allowed for the immunolocalization of nidogen-1 (Kostka et al, 2001; pmid:11564885).
Antibody First Published
Ries et al. Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies Eur J Biochem. 2001 Oct;268(19):5119-28. PMID:11589703
Note on publication
Recombinant proteins were produced of different predicted nidogen-1 domains in mammalian cells and used these proteins for crystallographic and binding epitope analyses.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
