Cat. No.
MABL-2393
Application
functional assay, SPR, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
JF4 (5G12-D4-E3)
From
Recombinant Antibody
Specificity
This antribody is specific for the G2 domain of nidogen-1. It binds in such a way that it is able to stop the binding of nidogen to laminin. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Alternative Names
Nidogen-1; Nid1; Entactin; NID-1
UniProt
P10493
Immunogen
This antibody was raised by immunizing a rat with he G2 region of mouse nidogen that is able to block its binding to laminin.
Application Notes
The binding of the mouse version of this antibody to nidogen-1 was proven by ELISA. Further, high binding affinity of the mouse version of this antibody to nidogen-1 was demonstrated using surface plasmon resonance (Ries et al, 2001; pmid:11589703). While researching nidogen in healthy human cartilage and in late-stage osteoarthritis cartilage an inhibition assay was preformed using the mouse version of this antibody. In short, wells were coated with either nidogen-1 or nidogen-2 to which this antibody was added. Then, chondrocytes were added and their adhesion to the nidogen was tested. 30-40% of chondrocytes showed adhesion to nidogen-1. (Kruegel et al, 2008; pmid:18438862). Tissue sections from Female New Mexico Research Institute (NMRI) mice were stained for immunohistochemistry using this antibody as a positive control.
Antibody First Published
Ries et al. Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies Eur J Biochem. 2001 Oct;268(19):5119-28. PMID:11589703
Note on publication
Recombinant proteins were produced of different predicted nidogen-1 domains in mammalian cells and used these proteins for crystallographic and binding epitope analyses.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
