Cat. No.
MABL-2412
Application
ICC, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
VU-1D9
From
Recombinant Antibody
Specificity
This antibody is specific for MUC-1
Alternative Names
Ep-CAM; EGP; EGP314; hEGP314; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; Epithelial cell adhesion molecule
UniProt
P16422
Immunogen
The original antibody was raised by immunizing a mouse with small cell lung carcinoma cell line H69.
Application Notes
The original version of this antibody was used for immunohistochemistry on ovarian cancer tissue arrays (Schaner et al., 2003; PMID:12960427), and on a variety of benign and malignant skin appendage tumors (Tsubura et al., 1992; PMID:1556271), and on squamous cell carcinoma (Dasgeb et al., 2013; PMID:24179394). This antibody was also used for immunocytochemistry on circulating tumor cells (Becker et al., 2013; PMID:24122526). The specificity of this antibody was determined using a western blot on K562 and MDAMB231 cell lines (Kershaw et al., 2015; PMID:25957999).
Antibody First Published
Tsubura et al. Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors J Cutan Pathol. 1992 Feb;19(1):73-9. PMID:1556271
Note on publication
The original paper studies Sixty-six benign and malignant skin appendage tumors for the expression and localization of the glycopreiteins identified by the monoclonal antibodies (MoAbs) GGDFP-15, GUI8, B72,3, and VU-1D9.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
