Cat. No.
MABL-2417
Application
functional assay, RIA, therapeutic, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AR20.5
From
Recombinant Antibody
Specificity
This antibody is specific for human MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Alternative Names
Mucin-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut- reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227
UniProt
P15941
Immunogen
This antibody was raised by immunizing BALB/c mice with MUC1.
Application Notes
While creating and characterizing this antibody, an ELISA was preformed on MUC1 and a 23-mer MUC1 peptide, E23 (CPAHGVTSAPDTRPAPGSTAPPA), Furthermore, the specificity of the antibody was confirmed using flow cytometry on MCF-7, BT-20 and 413BCR cell lines using the murine version of this antibody. Further, a chromium release assay was preformed using the murine version of this antibody on ZR75-1 cells. And finally the biodistribution of the mouse version of this antibody was tested. This was done on mice injected with ZR75-1 cancer cells (Qi et al, 2001; pmid:11839249). To assess the ability of this antibody to cure cancer mice inoculated with a Panc02.MUC1 cancer cell line were treated with the mouse version of this antibody.
Antibody First Published
Qi et al. Characterization of an anti-MUC1 monoclonal antibody with potential as a cancer vaccine. Hybrid Hybridomics. 2001;20(5-6):313-24. PMID:11839249
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
