Cat. No.
MABL-2416
Application
SPR, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
12D10
From
Recombinant Antibody
Specificity
This antibody is specific for the unsubstituted O-6 position of the Neu5Ac residue of MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T- cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53- mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Alternative Names
Mucin-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut- reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227
UniProt
P15941
Immunogen
This antibody was raised by immunizing female Balb/c mice with MUC1 antigen.
Application Notes
To characterize this antibody an ELISA was preformed on MUC1 glycorproteins. Furthermore, the binding constant of this antibody was determined by surface plasmon resonance on MUC1 glycoprotein using the mouse version of this antibody. Finally, human breast cancer cells (T-47D), human lung adenocarcinoma cells (Calu-3), human mucoepidermoid carcinoma cells (H292), and human lung adenocarcinoma epithelial cells were used for flow cytometry with the murine version of this antibody (Naito et al, 2017; pmid:30023556).
Antibody First Published
Naito et al. Generation of Novel Anti-MUC1 Monoclonal Antibodies with Designed Carbohydrate Specificities Using MUC1 Glycopeptide Library. ACS Omega. 2017 Nov 30;2(11):7493-7505. PMID:30023556
Note on publication
In this study, using an MUC1 glycopeptide library, we established two novel anti- MUC1 monoclonal antibodies (1B2 and 12D10) with designed carbohydrate specificities.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
