Cat. No.
MABL-2415
Application
ICC, IP, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Cat, Chicken, Pig, Primate, Rabbit, Rat, Human, Mouse
Clone No.
4-5M1
From
Recombinant Antibody
Specificity
This body is specific for the 5' terminal epitope of the MUC5AC.
Alternative Names
45M1; Mucin-5AC; Gastric mucin; Major airway glycoprotein; Tracheobronchial mucin; Mucin-5 subtype AC, tracheobronchial
UniProt
P98088
Immunogen
The original antibody was raised by immunizing mice with human gastric mucin M1.
Application Notes
The original version of this antibody was used for ELISA on NCI-H292 human pulmonary mucoepidermoid cells (Baginski et al., 2006; PMID:16543607), on BAL fluid (bronchoalveolar lavage fluid) (Phillips et al., 2006; PMID:16507438), and on different fractions of corpus tissue (Nordman et al., 2002; PMID:11988092). This antibody was also used for flow cytometry on human lung cells (Weeden et al., 2017; PMID:28125611), and on H292 cells (Montalbano et al., 2014; PMID:24797786). It was able to stain MUC5AC in both experiments. This antibody was able to stain passage 2 normal human tracheobronchial epithelial (NHTBE) cells for immunofluorescence, it was able to determine MUC5AC production during this experiment (Gray et al., 2004; PMID:14527933). The antibody was also used to stain HT29–18N2 goblet cells in a immunocytochemistry experiment, showing staining of the intracellular mucin stores (Stanley and Phillips., 1999; PMID:10409167). Immunoprecipitation on a mucin solution was performed using this antibody, allowing MUC5AC to be separated from the solution (Round et al., 2007; PMID:17356062). Finally, this antibody was used for a western blot on different recombinant versions of the MUC-5AC protein. showing that it bound specifically to the C-terminus of MUC-5AC (Lidell et al., 2008; PMID:18167142).
Antibody First Published
Stanley and Phillips. Selective secretion and replenishment of discrete mucin glycoforms from intestinal goblet cells Am J Physiol. 1999 Jul;277(1):G191-200. PMID:10409167
Note on publication
This original paper studies the effect of cholinergic stimulation on the mucin secretion of goblet cells
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
