Cat. No.
MABL-2414
Application
ICC, IRA, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2-12M1
From
Recombinant Antibody
Specificity
This antibody is specific for the C-terminus of MUC-5AC.
Alternative Names
Mucin-5AC; Gastric mucin; Major airway glycoprotein; Tracheobronchial mucin; Mucin-5 subtype AC, tracheobronchial
UniProt
P98088
Immunogen
The original antibody was raised by immunizing BALB/c mice with preparations of M1 antigens isolated from ovarian mucinous fluid of a pure endocervical type from an ALe(a-b+) patient.
Application Notes
The original version of this antibody was used for a western blot on different recombinant versions of the MUC-5AC protein (Lidell et al., 2008; PMID:18167142), on mucin proteins obtained from whole human stomachs (Mall et al., 1999; PMID:10343135), and on mucin proteins (Bara et al., 1988; PMID:2460087). This antibody was also used to stain MUC5AC in immunofluorescence on COS-7 cells transfected with pSecTag2 carrying fragments of the 59 end cDNA of MUC5AC (Nollet et al., 2004; PMID:15165482), and on BxPC-3, HT-29, LS174T, MCF-7, Calu-3 and Capan-1 cells (Liu et al., 2015; PMID:25595893). Furthermore this antibody was also used for immunoradiometric staining on fractions obtained during protein purification (Bara et al., 1991; PMID:1988372), and on a colon mucin -preparation of an 0 Le(a-b-) patient (Bara et al., 1988; PMID:2460087). Additionally this antibody was used for an ELISA on a M1 antigen preparation. And finally, this antibody was used for immunohistochemistry to stain the M1 antigen in fetal colonic mucosae (Bara et al., 1986; PMID:3524800).
Antibody First Published
Bara et al. Monoclonal antibodies against oncofetal mucin M1 antigens associated with precancerous colonic mucosae Cancer Res. 1986 Aug;46(8):3983-9. PMID:3524800
Note on publication
The original paper raises seven monoclonal antibodies (MAb) against a gastric mucin of an ALeb patient.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
