Cat. No.
MABL-2442
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
2D6-B1
From
Recombinant Antibody
Specificity
This antibody is specific for MF1, a fragment spanning amino acids 1 - 562 in the S1 domain of the head region of murine cardiac myosin. This antibody cross-reacts with keratin and GlcNAc-BSA.
Alternative Names
Q02566; Q91Z83; Q61879; Q6URW6; O08638; P13542; P13541; P97434; A2AQP0; Q02566
UniProt
Immunogen
This antibody was raised by immunising DBA/2 mice with cardiac myosin purified from BALB/c mouse hearts.
Application Notes
The reactivity of this antibody with mouse cardiac myosin and GlcNAc-BSA has been confirmed in ELISA analysis (Malkiel et al, 2000).The affinity of this antibody for myosin is higher than its affinity for GlcNAc-BSA (Malkiel et al, 2000). Administration of this antibody induces autoimmune myocarditis in DBA/2 mice, but not BALB/c mice (Liao et al, 1995). Equally, in immunohistochemical analysis, this antibody has been shown to be deposited throughout the hearts of DBA/2 mice, but not BALB/c mice (Liao et al, 1995).
Antibody First Published
Liao et al. Antibody-mediated autoimmune myocarditis depends on genetically determined target organ sensitivity The Journal of Experimental Medicine, 1995, Vol.181(3), p.1123-1130 PMID:7869033
Note on publication
Describes the original generation of this antibody, its characterisation, and its administration to DBA/2 mice to induce autoimmune myocarditis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.