Cat. No.
MABL-2462
Application
Activate, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
H2 scFv
From
Recombinant Antibody
Specificity
This scFv binds specifically to the fibronectin type III repeats 1–2 of mouse L1 with a KD of around 2μM (determined by ELISA). It does not cross-react with human L1 in ELISAs, Western blots or immunofluorescence staining of cultured neurons. L1 plays important roles in cell adhesion, neuronal migration, neurite outgrowth, fasciculation and pathfinding, neuronal survival, and synaptic plasticity. L1 is not recongised under reducing conditions. L1 is a membrane glycoprotein of the immunoglobulin superfamily. Its interacts with adhesion molecules and proteins of the ECM. L1 also is inolved in the migration of cerebellar granule cells, enhancing neuronal survival and memory.
Alternative Names
CD171; L1; Neural cell adhesion molecule L1; N-CAM-L1; L1
UniProt
P11627
Immunogen
single-chain variable fragment antibodies against mouse L1 from a human synthetic phage display library.
Application Notes
This scFv can stimulate neurite outgrowth from cerebellar dorsal, rootganglion and motor neurons and also be used to enhance survival of cerevellar neurons by facilitating the clustering of L1. H2 can also be used for ELISA, WB and IF studies.
Antibody First Published
Dong et al. Single chain Fv antibodies against neural cell adhesion molecule L1 trigger L1 functions in cultured neurons. Mol Cell Neurosci. 2003 Feb;22(2):234-47. PMID:12676533
Note on publication
Describes the generation and function of scFvs against mouse L1 protein.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
