Cat. No.
MABL-2461
Application
CD, FPA, RIA, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
NC10.14
From
Recombinant Antibody
Specificity
This antibody specifically binds the hapten NC174, a trisubstituted guanidinium sweetener.
Alternative Names
N-(p-cyanophenyl)-N'-(diphenylmethyl)-N''-(carboxymethyl)guanidine; N- (P-CYANOPHENYL)-N'-DIPHENYLMETHYL-GUANIDINE-ACETIC ACID; N-(pcyanophenyl)-N'-(diphenylmethyl)guanidine acetic acid; Trisubstituted guanidine sweetener; TGS
UniProt
Immunogen
The original antibody was generated by murine hybridomas produced by traditional techniques; mice were immunized with TGS covalently conjugated to bovine serum albumin carrier protein through one of the phenyl rings in Freund's complete adjuvant.
Application Notes
Ligand-induced quenching of IgG NC10.14 was used to determine the dissociation constant of the NC10.14-NC174 complex via IF and Circular Dichroism (CD) Spectroscopy (Viswanathan et al., 1996; PMID: 8756519). This antibody was used to study the ligand specificity of the putative sweet taste receptor binding site. Furthermore, the Fab of this antibody was used to show that aromatic-aromatic contacts at the interdomain surfaces are essential in the origination of CD spectra for both the near- and far- UV region (Tetin et al., 1996; PMID: 8573581). This antibody was examined to determine molecular recognition specificities using 14 different sweetener analogs in a competitive radioimmunoassay (Anchin et al., 1997; PMID: 9587873).
Antibody First Published
Anchin et al. Variable region sequence and characterization of monoclonal antibodies to a N,N',N"-trisubstituted guanidine high potency sweetener Mol Immunol. 1993 Nov; 30(16):1463-71. doi: 10.1016/0161-5890(93)90108-n PMID:8232332
Note on publication
The original publication explores the creation and characterization of a monoclonal antibodies library that targets a trisubstituted guanidinium sweetener.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
