Cat. No.
MABL-2469
Application
HPTLC, in vivo, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
P3
From
Recombinant Antibody
Specificity
This antibody is specific for the N-glycolyl neuraminic acid (NeuGc) linked to the internal galactose of gangliosides, showing no cross-reactivity to the highly similar N-acetylated (NeuAc) gangliosides that are common cellular antigens in humans. This antibody was shown to react strongly with NeuGcGM3 and NeuGcGM2, but not with GM3, GM2, GM1, GD1a, GD1b, GD3, GD2, GT1b, Gg4Cer, or LacCer. This antibody also binds sulfated glycolipids.
Alternative Names
NeuGcGM2; N-glycolyl neuraminic acid-containing gangliosides; NeuGc- containing gangliosides; GM3(NeuGc) ganglioside
UniProt
Immunogen
The original antibody was generated by immunizing Balb/c mice with NeuGcGM3 liposomes.
Application Notes
The original antibody (mouse IgM) was generated and screened via ELISA against for gangliosides of interest. This antibody was used for tumor recognition in IHC, showing a strong positive reaction in ductal infiltrating breast carcinoma, breast cancer metastasis in lymph nodes, and breast adenosis tissue, with no cross-reactivity to other malignant or benign tumors. Additionally, it was used in in vivo experiments with Balb/c mice to stimulate immune responses, generating a significant IgG-type anti- idiotypic (Ab2) response, which increased when conjugated to KLH and administered with adjuvants. This antibody also produced an anti-anti-idiotypic (Ab1') response, demonstrating its ability to connect to both T and B cell networks and generate a specific antigen-independent immune response (EP0657471B1). This antibody was used as a positive control in an in vitro and in vivo study (Carr et al., 2002; PMID: 12573110). A chimeric(humanized; human IgG1) variant of this antibody was created (chP3), and the crystal structure of its Fab was solved (Talavera et al., 2009; PMID: 19748674).
Antibody First Published
Vázquez et al., Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids Hybridoma. 1995 Dec;14(6):551-6. doi: 10.1089/hyb.1995.14.551. PMID:8770642
Note on publication
The original publication describes the generation of the P3 murine monoclonal antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
