Cat. No.
MABL-2475
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
12H2
From
Recombinant Antibody
Specificity
This antibody recognizes human α4β2 receptor protein. The AChR responds by an extensive change in conformation after binding acetylcholine, that affects all subunits and leads to opening of an ion- conducting channel across the plasma membrane permeable to sodium ions.
Alternative Names
CHRNB2; Nicotinic acetylcholine α4β2 receptor; Neuronal acetylcholine receptor subunit beta-2; human α4β2 nicotinic receptor; AChR
UniProt
P17787
Immunogen
The original antibody was generated by immunizing female BALB/c mice with human α4β2 receptor protein purified in n-dodecyl-β-D-maltopyranoside (DDM).
Application Notes
The binding characterization of this antibody to human α4β2 nicotinic receptor was done using ELISA.
Antibody First Published
Walsh et al. Structural principles of distinct assemblies of the human α4β2 nicotinic receptor. Nature. 2018 May;557(7704):261-265. PMID:29720657
Note on publication
This paper investigates the structural basis of the distinctive biophysical and pharmacological properties of the two different stoichiometries of this receptor.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
