Cat. No.
MABL-2476
Application
SPR, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
JF5 (6E4-C9-B7)
From
Recombinant Antibody
Specificity
This antribody is specific for the G2 domain of nidogen-1. It binds in such a way that it is able to stop the binding of nidogen to laminin. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Alternative Names
Nidogen-1; Nid1; Entactin; NID-1
UniProt
P10493
Immunogen
This antibody was raised by immunizing a rat with he G2 region of mouse nidogen that is able to block its binding to laminin.
Application Notes
The binding of the mouse version of this antibody to nidogen-1 was proven by ELISA. Further, high binding affinity of the mouse version of this antibody to nidogen-1 was demonstrated using surface plasmon resonance (Ries et al, 2001; pmid:11589703). To compare nidogen-1 to nidogen-2 in their ability to bind basement membrane components, tissues from embryotic and adult mice were stained using the mouse version of this antibody to be used for immunohistochemistry (Salmivirta et al, 2002; pmid:12243745).
Antibody First Published
Ries et al. Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies Eur J Biochem. 2001 Oct;268(19):5119-28. PMID:11589703
Note on publication
Recombinant proteins were produced of different predicted nidogen-1 domains in mammalian cells and used these proteins for crystallographic and binding epitope analyses.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
