Cat. No.
MABL-2478
Application
antagonist, Block, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Z199
From
Recombinant Antibody
Specificity
This antibody binds the human NKG2a. It can also recognize and bind the CD94/NKG2a heterodimer formed by the covalent association of CD94 with the NKG2a molecule. The CD94/NKG2a heterodimer acts as an inhibitory natural killer cell receptor and binds nonclassical MHC class I protein, HLA-E. NKG2a is found mainly on NK cells and on subsets of CD8+ cells.
Alternative Names
CD159a; NKG2-A; NKG2a; KLRC1; NK cell receptor A; CD159 antigen-like family member A; NKG2-A/NKG2-B type II integral membrane protein; NKG2-A/B-activating NK receptor
UniProt
P26715
Immunogen
Application Notes
This antibody was reported to be a non-competitive antagonist of human CD94/NKG2A receptor expressed on lymphocytes, since it inhibited the function of CD94/NKG2A. Z199 can block the function of human CD94/NKG2A, and specifically induce killing of cells by CD94/NKG2A-restricted lymphocytes in a concentration-dependent fashion (US8796427). This antibody was used to study the covalent interactions between CD94 and NKG2A, which together form the inhibitory natural killer cell receptor (PMID: 9045931). This antibody was used to study the binding interactions of HLA-E (wild-type) tetramer to CD94/NKG2A or CD94/NKG2C expressing cells by flow cytometry (PMID: 14971033). This antibody was used to test the cytotoxic activity of NKL cells against tumor cells. NKL cells were tested for their ability to lyse the indicated targets in a 51Cr release assay, this antibody was found to enhance NK lysis (PMID: 21969815).
Antibody First Published
Carretero et al. The CD94 and NKG2-A C-type lectins covalently assemble to form a natural killer cell inhibitory receptor for HLA class I molecules. Eur J Immunol. 1997 Feb;27(2):563-7. PMID:9045931
Note on publication
Describes the use of this antibody to study the covalent interactions between CD94 and NKG2-A.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
