Cat. No.
MABL-2486
Application
agonist, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
cynomolgous monkey, Human, Rhesus Monkey
Clone No.
ON72
From
Recombinant Antibody
Specificity
This antibody recognizes human, cynomolgous monkey and rhesus monkey NKG2D, but does not cross react with mouse rat, dog or pig NKG2D. Natural killer (NK) cells serve as key innate effectors and their activity has been considered a prognostic biomarker in diverse human diseases. NKG2d functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. It provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. I also acts as a costimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. and participates in NK cell-mediated bone marrow graft rejection.
Alternative Names
KLRK1; CD314; NKG2-D type II integral membrane protein; NK cell receptor D; Killer cell lectin-like receptor subfamily K member 1; NKG2-D-activating NK receptor; KYK2; KYK- 2.0
UniProt
P26718
Immunogen
The original antibody was generated by grafting of CDRs from mouse parental clone onto human framework regions.
Application Notes
This antibody is capable of recognizing NKG2D in an ELISA. This antibody was successful in staining and identification NKG2D expressed on PBMCs isolated from human, cynomolgous monkey and rhesus monkey in a flow cytometric assay (US7879985). This antibody was used to study the distribution of NKG2D on human peripheral blood cells by flow cytometry (PMID: 15048706). The immobilized version of this antibody was reported to show agonistic activity (US7879985).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
