Cat. No.
MABL-2495
Application
in vitro, ELISA
Isotype
Engineer antibody
Species Reactivity
Porcine reproductive and respiratory syndrome virus
Clone No.
Nb43
From
Recombinant Antibody
Specificity
The antibody is specific for Nsp4.
Alternative Names
NSP4; Replicase polyprotein 1ab; ORF1ab polyprotein
UniProt
Q9YN02
Immunogen
An adult male Bactrian camel was immunized subcutaneously with the purified Nsp4-NHis recombinant protein. A camel VHH library was then constructed and panned against Nsp4.
Application Notes
The specificity of the original format of the antibody was confirmed by indirect ELISA analysis. MARC-145 cell lines expressing nanobodies were generated and infected with PRRSV strain SD16. At 24 h post-infection the antibody inhibited infectious virus release by about 99%. Further, the antibody protected MARC-145 cells from any virus-induced cytopathic effect and fully blocked PRRSV replication (Liu et al. 2016; PMID: 27010387).
Antibody First Published
Liu et al. Intracellularly expressed nanobodies against non-structural protein 4 of porcine reproductive and respiratory syndrome virus inhibit virus replication Biotechnol Lett. 2016 Jul;38(7):1081-8. PMID:27010387
Note on publication
The original paper describes the generation and characterization of a set of antibodies by phage display method.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
