Cat. No.
MABL-2504
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
E6
From
Recombinant Antibody
Specificity
An engineered ScFv fragment antibody that binds to both mouse mNRR1 and human hNRR1 but not NRR2.
Alternative Names
Tan1; Notch1; Mis6; N1; lin-12; NOTCH1; TAN1; hN1
UniProt
P46531 (human); Q01705 (mouse)
Immunogen
This antibody was selected against a fusion protein consisting of EGF domains 1–12 of murine Notch1 fused to a human Fc domain and binds to the NRR of mouse Notch1.
Application Notes
Antibody First Published
Falk R, Falk A, Dyson MR, Melidoni AN, Parthiban K, Young JL, Roake W, McCafferty J. Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods. 2012 Sep;58(1):69-78. PMID:22842086
Note on publication
Describes the generation of Notch receptor blocking antibodies using Phage Display.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
