Cat. No.
MABL-2505
Application
Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
N248A
From
Recombinant Antibody
Specificity
This antibody binds to notch 1 at two distinct sets of epitopes, one in Lin-A domain and other in HD-C domain (aa 1705- 1710), causing the notch 1 to be locked down in the silent conformation, and thus preventing the receptor from being activated by its ligands.
Alternative Names
Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1
UniProt
P46531
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant notch 1 fusion protein as immunogen. The fusion protein contained N-terminal leader peptide, EGF-like repeats 35 to 36, NRR (including Lin A, B and C domains and the HD domain) and a small portion of intracellular sequence.
Application Notes
This antagonist antibody binds notch 1 with an affinity of Kd= 0.33 nM. The binding epitopes of Notch 1 antibody was analyzed by domain swap and ELISA binding assays. mAb N248A inhibits both T-cell acute lymphoblastic leukemia (T-ALL) and breast cancer cell growth in cell culture. N248A also inhibits T-cell lymphoblastic leukemia in murine xenograft tumor model (WO2012080891).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
