Cat. No.
MABL-2510
Application
ELISA;
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
GLA-S4F18
From
Recombinant Antibody
Specificity
This antibody is capable of binding both human and mouse notch 4. Ths binding epitope of this antibody is located in the negative regulatory region or the NRR.
Alternative Names
hNotch4; Neurogenic locus notch homolog protein 4
UniProt
Q99466
Immunogen
The original antibody was isolated from a phage display library generated from a non immunized donor and panning against human and mouse Notch4-negative regulatory region or NRR antigens.
Application Notes
The original human IgG1, GLA-S4F18 antibody binds human and mouse notch 4 with an EC50 of 0.09 nM and 0.11 nM respectively as measured by direct ELISA. The binding of this antibody to surface expressed notch 4 was determined by flow cytometry. This antibody was reported to significantly delay tumor regrowth when administered in combination with carboplatin in an ovarian xenograft tumor model. When used in combination with cisplatin it delayed tumor regrowth in ovarian PDX models. It also resulted in reduction in cancer stem cells (CSC) present in tumors when treated with GLA-S4F18 alone or in combination with abraxane (WO2018039107).
Antibody First Published
WO2018039107
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
