Cat. No.
MABL-2524
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
JC1
From
Recombinant Antibody
Specificity
This antibody detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
Alternative Names
UniProt
Immunogen
This antibody was raised in mice against Pokeweed-stimulated lymphocytes of human origin.
Application Notes
This antibody might be used in determining the rate of proliferation in cancer cells. During immunohistochemical stainings, JC1 antibody has been demonstrated to recognise a nuclear marker specific for proliferating cells (both normal and tumour) and distinct from Ki67 (Garrido et al. 1992). Importantly, the advantage of this antibody is that it does not present any additional cytoplasmic labelling in squamous or other cells (as it is the case with Ki67) (Garrido et al., 1992). Furthermore, JC1 antibody was shown to work in Western blot analysis labelling two bands in a sample derived from IM-9 cell lysates (Garrido et al., 1992). The bands were, again, different than those for Ki67, what presents an opportunity of utilising JC1 antibody as an independent and parallel clinical assessment of proliferation next to Ki67.
Antibody First Published
Garrido et al. Monoclonal antibody JC1: new reagent for studying cell proliferation. J Clin Pathol. 1992 Oct;45(10):860-5. PMID:1430255
Note on publication
This article describes characterisation of the JC1 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
