Cat. No.
MABL-2528
Application
IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
NA24
From
Recombinant Antibody
Specificity
This antibody is specific towards nucleophasmin which is a widely expressed phosphorylated protein involved in various processes, such as ribosome assebly and transport, cell proliferation, protein chaperoning and centrosome duplication. Aberrations involving nucleophasmin are found in multiple conditions, for instance in a form of non-Hodgkin lymphoma or acute promyelocytic leukemia.
Alternative Names
NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; B23; NPM1; Nucleolar protein NO38
UniProt
P06748
Immunogen
This antibody was raised by immunising BALB/C mice with GST fusion protein containing the N-terminal part of nucleophosmin fused to 14 amino acid of ALK protein.
Application Notes
Mutations associated with nucleophasmin are present in varous conditions and this antibody has proven useful in different experiments investigating their molecular underpinnings. One group used NE24 antibody to perform immunohistochemical staining confirming that anaplastic large-cell lymphoma involves expression of mutated NPM-ALK fusion (Cordell et al., 1999). Finally, alterations of nucleophasmin expression were found in many cancers where this antibody might be utilised to analyse them. For example, Gimenez and colleagues (2010) performed immunohistochemical stainings and Western blot analyses to demonstrate aberrations in nucleophasmin expression in brain cancer.
Antibody First Published
Cordell et al. Detection of normal and chimeric nucleophosmin in human cells. Blood. 1999 Jan 15;93(2):632-42. PMID:9885226
Note on publication
This article describes generation and characterisation of NA24 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
