Cat. No.
MABL-2540
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4155
From
Recombinant Antibody
Specificity
The antibody binds to E3G with an affinity of 10 nM.
Alternative Names
E3G
UniProt
Immunogen
Human E3G.
Application Notes
The antibody is specific for Oestrone-3-glucuronide (E3G), a urinary metabolite of 17β- estradiol, a hormone secreted by the developing ovarian follicles. The monoclonal antibodies can be used clinically to detect levels of E3G in urine samples of women, providing a non-invasive test for determining the fertile period, indicating the time of ovulation. The antibody also has a lower affinity (1 nM) for estriol 3- (β-D-glucuronide), which differs to E3G by a single hydroxyl group.
Antibody First Published
Trinh CH, Hemmington SD, Verhoeyen ME, Phillips SE. Antibody fragment Fv4155 bound to two closely related steroid hormones: the structural basis of fine specificity. Structure. 1997 Jul 15;5(7):937-48. PMID:9261086
Note on publication
Describes the production the monoclonal antibody 4155, which can be clinically useful in the detection of steroid glucuronides concentrations in samples of early morning urine, indicating the fertile period in the females menstrual cycle.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
