Cat. No.
MABL-2542
Application
ICC, therapeutic, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse, Rat
Clone No.
sHIgM46 (LYM46)
From
Recombinant Antibody
Specificity
This antibody recognizes and binds an epitope on the cell surface of human oligodendrocytes and central white matter of the cerebellum. This antibody also cross reacts with antigens present on mouse and rat oligodendrocytes.
Alternative Names
oligodendrocyte
UniProt
Immunogen
The original antibody was isolated from the sera obtained from 102 patients with a wide variety of conditions including Waldenstrom’s macroglobulinemia, multiple myeloma, lymphoma, and monoclonal gammopathy.
Application Notes
This antibody is directed against the cell surface antigen of oligodendrocyte and promotes significant remyelination in a virus mediated model of multiple sclerosis (MS). A single 0.5 mg peripheral dose of either of two human IgMs (sHIgM22 and sHIgM46) promoted significant remyelination in chronically Theiler’s murine encephalomyelitis virus (TMEV) infected mice (PMID: 10841576). This antibody was used in the immunohistochemical staining of 8 week SJL/J mouse cerebellar slices and was found to bind the white matter the internal granular layer, Purkinje cell soma, and small cells within the molecular layer (PMID: 15198125). This antibody was also used in the immunohistochemical and immunocytochemical analysis of active MS lesions from 83 biopsies and autopsies of MS patients and was also used in the identification of four different pathologic subtypes of active MS lesions (PMID: 21961063)
Antibody First Published
Warrington et al. Human monoclonal antibodies reactive to oligodendrocytes promote remyelination in a model of multiple sclerosis. Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6820-5. PMID:10841576
Note on publication
Describes the generation of two antibodies that promote remyelination in a virus mediated mouse model of multiple sclerosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
