Cat. No.
MABL-2543
Application
functional assay, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D5 (D5E)
From
Recombinant Antibody
Specificity
This antibody recognizes the early-stage oligomeric morphological form of human alpha- synuclein. Alpha-synuclein is a small 140 amino acid presynaptic neuronal protein, a major component of Lewy bodies and a member of the synuclein family. Misfolding, abnormal accumulation, and secretion of α- Synuclein (α-Syn) are closely associated with synucleinopathies, including Parkinson’s disease (PD). α-syn is abundant in the brain, and smaller amounts are found in the heart, muscles, and other tissues. In the brain, α-syn is found mainly at the tips of neurons in the specialized structures known as presynaptic terminals and has been shown to comprise up to ∼1% of total proteins in the neuronal cytosol. Recent evidence suggests that α-synuclein (α-syn) can contribute to the pathogenesis of amyotrophic lateral sclerosis (ALS).
Alternative Names
SNCA; NACP; PARK1; alpha-syn; α-Synuclein; α-syn; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; oligomeric α-synuclein; oligomeric α- syn
UniProt
P37840
Immunogen
The original antibody was isolated from a human Tomlinson I and J scFv antibody libraries by panning the library against oligomeric α-synuclein immobilized on a mica surface. The presence of positive binding phage after each round was verified by incubating an aliquot of eluted phage with α-synuclein and imaging by Atomic Force Microscopy (AFM).
Application Notes
The binding of this antibody to oligomeric forms of alpha-synuclein was confirmed using ELISA. This antibody is not capable of recognizing monomeric and fibrillar forms of alpha-synuclein. This antibody could also recognize molecular masses of about 29 and 56 kDa corresponding to dimeric and tetrameric forms of alpha-syn form in a western blot (PMID： 17391701; 19141614). The scFv form of this antibody binds only to an oligomeric form of α-synuclein and inhibits both aggregation and toxicity of α-synuclein in vitro (PMID: 17391701). This antibody could recognize the early stage alpha-synuclein aggregates occurring during (4–10 day) time points (PMID: 19141614). Intracellular expression of scFv version of this antibody containing a non-conventional secretion signal sequence could successfully bind their cytosolic targets and secrete them from a mammalian cell.
Antibody First Published
Emadi Isolation of a human single chain antibody fragment against oligomeric α-synuclein that inhibits aggregation and prevents α-synuclein induced toxicity J Mol Biol. 2007 May 11; 368(4): 1132–1144. PMID:17391701
Note on publication
Describes the isolation of antibody fragments that bind to a specific morphology of alpha-synuclein using phage display antibody technology and atomic force microscopy (AFM).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
