Cat. No.
MABL-2544
Application
functional assay, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
10H (syn-10H)
From
Recombinant Antibody
Specificity
This antibody recognizes the later-stage oligomeric morphological form of human alpha- synuclein. Alpha-synuclein is a small 140 amino acid presynaptic neuronal protein, a major component of Lewy bodies and a member of the synuclein family. Misfolding, abnormal accumulation, and secretion of α- Synuclein (α-Syn) are closely associated with synucleinopathies, including Parkinson’s disease (PD). α-syn is abundant in the brain, and smaller amounts are found in the heart, muscles, and other tissues. In the brain, α-syn is found mainly at the tips of neurons in the specialized structures known as presynaptic terminals and has been shown to comprise up to ∼1% of total proteins in the neuronal cytosol. Recent evidence suggests that α-synuclein (α-syn) can contribute to the pathogenesis of amyotrophic lateral sclerosis (ALS).
Alternative Names
SNCA; NACP; PARK1; alpha-syn; α-Synuclein; α-syn; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; oligomeric α-synuclein; oligomeric α- syn
UniProt
P37840
Immunogen
The original antibody was isolated from a human Tomlinson I and J scFv antibody libraries by panning the library against diluted sample of alpha-synuclein (20 uM) containing predominantly larger aggregate forms.
Application Notes
The binding of this antibody to oligomeric forms of alpha-synuclein was confirmed using ELISA. This antibody is not capable of recognizing monomeric and fibrillar forms of alpha-synuclein. This antibody could recognize the late stage alpha-synuclein aggregates occurring during (10–22 day) time points. It was capable of binding aggregates with molecular masses of 42 and ~80 kDa corresponding to trimeric and hexameric forms in a western blot. The scFv version of this antibody inhibits aggregation of alpha-synuclein in vitro, blocks extracellular alpha-synuclein-induced toxicity in both undifferentiated and differentiated human neuroblastoma cell lines (SH-SY5Y), and specifically recognizes naturally occurring aggregates in Parkinson's disease but not in healthy human brain tissue (PMID: 19141614). A fusion protein comprising D5 scFv, cell pentrating peptide like penetratin were found to be effective in Lewy body disease. It specifically targeted α-syn oligomers and reduced the accumulation of α-syn and ameliorated functional deficits when delivered late in disease development (PMID: 27606342).
Antibody First Published
Emadi et al. Detecting Morphologically Distinct Oligomeric Forms of α- Synuclein. J Biol Chem. 2009 Apr 24; 284(17): 11048–11058. PMID:19141614
Note on publication
Describes the isolation of antibody fragments that bind to a specific morphology of alpha-synuclein using phage display antibody technology and atomic force microscopy (AFM).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
