Cat. No.
MABL-2548
Application
inhibition, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AT
From
Recombinant Antibody
Specificity
The antibody recognizes Human OPGbp (residues 143-317). OPGbp acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. It inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro.
Alternative Names
Osteoprotegerin binding protein; Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin
UniProt
O00300
Immunogen
The original antibody was generated by panning against a human Fab library.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding affinity of the Fab and IgG1 fragments of the antibody to OPGbp was measured by surface plasmon resonance (Kd≈ 140 nM and 0.33-0.43 nM respectively). The interaction of human OPGbp and its receptor ODAR was measured in a fluorescence energy transfer (FRET) assay. The Fab fragment of the antibody inhibited OPGbp binding to soluble ODAR-Fc fusion with an IC50 of 550 nM. Bone Marrow Assay using the Fab fragment of the antibody gave a 50% decrease (IC50) at 57.8 nM. In RAW Cell Assay the Fab fragment of the antibody showed an IC50 of 15 μg/ml, 300 nM. In RAW Cell Assay, the 3 full length versions of the antibody “AT”405, “AT”406 and “AT”407 showed IC50 of 20.1 nM, 60.3 nM & 21.4 nM, respectively. “AT”405 or “AT”407 were tested in murine bone marrow assay, showing IC50 of 2.15 ug/ml (14.4 nM), and 1.85 ug/ml (12.5 nM), respectively. “AT”406 was tested in the bone marrow coculture assay and inhibited TRAP formation with an IC50 of 2.35 μg/ml (14.4 nM) (US20140147444).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
