Cat. No.
MABL-2549
Application
in vivo, NTRL, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
18B2
From
Recombinant Antibody
Specificity
This antibody is specific for human OPGL. In vivo experiments have shown cross reactivity with Cynomolgus Monkey OPGL.
Alternative Names
CD254; Tumor necrosis factor ligand superfamily member 11; TNFSF11; TNF superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; OPTB2; Receptor activator of nuclear factor kappa-B ligand; RANKL; hRANKL2; TNF-related activation-induced cytokine; TRANCE; sOdf; TNLG6B
UniProt
O14788
Immunogen
The original antibody was generated by immunizing transgenic HuMab mice with purified recombinant OPGL derived from E. coli or CHO cells. Sera from immunized mice were tested for antibody binding to OPGL.
Application Notes
The binding affinity of this antibody's original format (human IgG1) to hOPGL was confirmed by ELISA. Its binding kinetics to hOPGL 140 and hOPGL 158 was measured by SPR analysis; a KD of 0.211 nM was measured for hOPGL 140. This antibody was evaluated for its neutralizing activity against osteoclast formation induced by Osteoprotegerin ligand (OPGL) using RAW 264.7 cells, as measured by tartrate-resistant acid phosphatase (TRAP) activity; it inhibited osteoclast formation in a dose-dependent manner with an IC50 of 80 ng/ml. This antibody's pharmacokinetics and in vivo activity were assessed in cynomolgus monkeys — three female cynomolgus monkeys (not greater than five years of age, each weighing between 2 to 5 kgs) received a single subcutaneous dose of 1 mg/kg; serum samples were collected over 56 days to determine the levels of the antibody and analyze bone turnover markers such as serum N-telopeptide (serum N-Tx). The antibody's levels in the serum declined slowly over the testing period; the last measurement on day 56 showed a serum concentration of ~100 ng/ml. Serum N-Tx levels were greatly reduced compared to baseline immediately following injection (~minus 60%), further decreased to a minimum of ~minus 75% over a period of two weeks, after which they began to increase gradually but remained lower in a statistically significant manner than baseline with a final measurement of ~minus 25% on day 56 (US7718776B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
