Cat. No.
MABL-2551
Application
crystallisation, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Borrelia burgdorferi
Clone No.
184.1
From
Recombinant Antibody
Specificity
184.1 was shown to react specifically with a common OspA epitope shared by a homologous protein of lower molecular weight (Jiang et al, 1990). Based on N-terminal sequencing and reactivity of 184.1 against truncated versions of OspA, it was concluded that 184.1 recognises an N-terminal epitope of OspA. This was corroborated by a crystal structure of the OspA-184.1 Fab structure (Li et al, 1997), which showed that 184.1 binds a well-ordered beta sheet arrangement near the N-terminus.
Alternative Names
Outer surface protein A
UniProt
P0A3N6
Immunogen
BALB/c mice were immunised by injection of B. burgdorferi sonicated preparations. Splenocytes were obtained from immunised mice and fused with X63-Ag8 myeloma cells to generate hybridomas.
Application Notes
184.1 was used in Western blots and ELISA to determine specificity against B. burgdorferi major surface proteins (Jiang et al, 1990).
Antibody First Published
Jiang et al. Cross-antigenicity between the major surface proteins (ospA and ospB) and other proteins of Borrelia burgdorferi. J Immunol. 1990 Jan 1;144(1):284-9. PMID:2295795
Note on publication
Describes the generation and use of mAbs against B. burgdorferi to characterise structural heterogeneity between the major surface proteins OspA and OspB.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
