Cat. No.
MABL-2552
Application
IP, NTRL, WB, IF
Isotype
Engineer antibody
Species Reactivity
Borrelia burgdorferi
Clone No.
LA-2
From
Recombinant Antibody
Specificity
Recognises the three surface-exposed loops of the C-terminal domain of OspA that are on the tip of the elongated molecule most distant from the lipid-modified N-terminus. The residues involved in these loops are: 203-220, 224-233, and 246-257.
Alternative Names
Outer surface protein A
UniProt
P0A3N6
Immunogen
This antibody was generated by immunising mice.
Application Notes
LA-2 binds to Borrelia Burgdorferi, the causative agent of Lyme disease (Borreliosis) has been used in serological analyses of B. burgdorferi isolates by immuno-blotting and immunofluorescence (Kramer et al, 1990). This antibody has also been applied to affinity purification of OspA. This antibody neutralised B. burgdorferi infectivity when passively transferred to SCID mice (Schaible et al, 1990).
Antibody First Published
Wilske et al. Antigenic Variability of Borrelia burgdorferi Ann N Y Acad Sci. 1988;539:126-43. PMID:2461135
Note on publication
Describes the use of monoclonal and polyclonal antibodies to characterise proteins of B. burgdorferi by Western Blot.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
