Cat. No.
MABL-2553
Application
IP, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Borrelia burgdorferi
Clone No.
H6831
From
Recombinant Antibody
Specificity
This antibody is directed against the C terminus of outer surface protein B (OspB), a major lipoprotein of the Lyme disease spirochete, Borrelia burgdorferi. This antibody binds an epitope topologically analogous to the LA-2 epitope of OspA and is centered around OspB Lys-253, a residue essential for recognition by H6831.
Alternative Names
Outer surface protein B
UniProt
P17739
Immunogen
The original antibody was generated by immunizing BALB/c mice with B31 strain of Borrelia burgdorferi.
Application Notes
Indirect immunofluorescence was used to determine the reactivity of this antibody towards 14 strains of Lyme disease spirochetes. This antibody was also used for the immunoprecipitation of Shelter Island LD spirochete isolate (PMID： 6735474). This antibody capable of detecting B. burgdorferi B311 cell lysates in a western blot. (PMID: 6735474; 7513309). This antibody has an unusual property of having bactericidal effect even in the absence of complement or phagocytes. In time-kill studies, more than 99.9% of strain B31 B. burgdorferi cells were killed after 30 min of exposure to H6831 Fab fragments. It was reported that the whole IgG version of this antibody inhibited B311 cells but did not inhibit the growth of Osp-less B313 cells even at 25 mg/ml (PMID: 7890424). ELISA was used to check the bactericidal activity of this antibody towards whole cells of B. burgdorferi strain B311 after incubation with serial dilutions of this antibody (PMID: 7513309). This antibody was also used to identify its usability in a capture ELISA for detecting various isolates of Borrelia burgdorferi (PMID: 8432812).
Antibody First Published
Barbour et al. Variation in a major surface protein of Lyme disease spirochetes. Infect Immun. 1984 Jul; 45(1): 94–100. PMID:6735474
Note on publication
Describes the generation of this antibody and mentions its use in the characterization of a surface antigen the contributes to the serotype specificity of Lyme disease spirochetes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
